The protective effects of hydrogen on HO-1 expression in the brainafter focal cerebral ischemia reperfusion in rats. | ラットの局所性脳虚血後の脳中のHO-1発現に対する水素の保護効果

Turk J Med Sci. 2016 Nov 17;46(5):1534-1539. doi: 10.3906/sag-1502-3.

The protective effects of hydrogen on HO-1 expression in the brainafter focal cerebral ischemia reperfusion in rats.

Department of Critical Care Medicine, Yu Huang Ding Hospital, Qingdao University

Abstract

BACKGROUND/AIM:

The aim of this study was to investigate whether a hydrogen administration can produce neuroprotective effects after brain ischemia reperfusion in rats.

MATERIALS AND METHODS:

A brain ischemia reperfusion injury was induced by a 2-h left middle cerebral artery occlusion (MCAO) using an intraluminal filament, followed by 46 h of reperfusion. A hydrogen-rich saline (1 mL/kg body weight i.p.) was administered at the beginning of reperfusion. Saline (1 mL/kg)-treated animals were used as the control. Sham-operated animals were also used. Subsequently, 48 h after the MCAO, histological alternations, heme oxygenase-1 (HO-1) expression, and levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the cerebral cortex and the hippocampus were examined.

RESULTS:

Hydrogen significantly alleviated brain tissue histological damage, promoted HO-1 expression, upregulated levels of SOD, and decreased the levels of MDA in brain tissue after the ischemia reperfusion injury.

CONCLUSION:

The results suggest that the neuroprotective effects of hydrogen may be mediated by promoting HO-1 expression and attenuated the oxidative injury.

Turk J Med Sci. 2016 Nov 17;46(5):1534-1539. doi: 10.3906/sag-1502-3.

ラットの局所性脳虚血後の脳中のHO-1発現に対する
水素の保護効果

青島大学 yu huang医院救命救急医療

＜要旨＞

背景／目的

本研究はラットの脳虚血再灌流に対して、水素投与が神経保護効果を発揮するかどうかを調べるために行いました。

方法

脳虚血再灌流障害モデルは、腔内フィラメントを用いて左中央脳動脈結索（MCAO）を2時間実施し、46時間後に灌流再開することで作成しました。高濃度水素生理食塩水（1mL/kgBW,i.p.）は再灌流開始時に投与しました。また、生理食塩水（1mL/kg）を投与されたラットをコントロールとし、別途シャム処置群も設けました。

MCAOから48時間後に、脳皮質や海馬における組織学的変性やHO-1発現、マロンジアルデヒド（MDA）・SODの値を測定しました。