Molecular hydrogen attenuates neuropathic pain in mice. | 水素分子はマウスの神経障害性疼痛を和らげる

PLoS One. 2014 Jun 18;9(6):e100352. doi: 10.1371/journal.pone.0100352. eCollection 2014.

Molecular hydrogen attenuates neuropathic pain in mice.

Department of Anesthesiology, National Defense Medical College


Neuropathic pain remains intractable and the development of new therapeutic strategies are urgently required. Accumulating evidence indicates that overproduction of oxidative stress is a key event in the pathogenesis of neuropathic pain. However, repeated intra-peritoneal or intrathecal injections of antioxidants are unsuitable for continuous use in therapy. Here we show a novel therapeutic method against neuropathic pain: drinking water containing molecular hydrogen (H2) as antioxidant. The effect of hydrogen on neuropathic pain was investigated using a partial sciatic nerve ligation model in mice. As indicators of neuropathic pain, temporal aspects of mechanical allodynia and thermal hyperalgesia were analysed for 3 weeks after ligation. Mechanical allodynia and thermal hyperalgesia were measured using the von Frey test and the plantar test, respectively. When mice were allowed to drink water containing hydrogen at a saturated level ad libitum after ligation, both allodynia and hyperalgesia were alleviated. These symptoms were also alleviated when hydrogen was administered only for the induction phase (from day 0 to 4 after ligation). When hydrogen was administered only for the maintenance phase (from day 4 to 21 after ligation), hyperalgesia but not allodynia was alleviated. Immunohistochemical staining for the oxidative stress marker, 4-hydroxy-2-nonenal and 8-hydroxydeoxyguanosine, showed that hydrogen administration suppressed oxidative stress induced by ligation in the spinal cord and the dorsal root ganglion. In conclusion, oral administration of hydrogen water may be useful for alleviating neuropathic pain in a clinical setting.

PLoS One. 2014 Jun 18;9(6):e100352. doi: 10.1371/journal.pone.0100352. eCollection 2014.


防衛医科大学校 麻酔科



神経障害性疼痛に対する水素の効果を、坐骨神経部分損傷モデルマウスを用いて調べました。神経損傷の施術後3週間にわたって、神経障害性疼痛の指標となる機械的アロディニアおよび温痛覚過敏について調べました。なお、機械的アロディニアについてはvon Freyテスト、温痛覚過敏はplantarテストを用いて評価しました。