Hydrogen-water enhances 5-fluorouracil-induced inhibition of colon cancer. | 水素水は5-FUによる結腸がんの抑制効果を促進する

PeerJ. 2015 Apr 7;3:e859. doi: 10.7717/peerj.859. eCollection 2015.

Hydrogen-water enhances 5-fluorouracil-induced inhibition of colon cancer.

Department of Psychosomatic Internal Medicine, Kagoshima University Graduate School of Medical and Dental Sciences


Oxidative stress is involved in cancer development. Hydrogen (H2) is a potent antioxidant and exhibits anti-inflammatory and potentially anticancer-like activities. This study aimed to investigate the role of H2 incombination with 5-fluorouracil (5-FU) in cancer treatment both in vitro and in vivo using the colon 26 cell line. The survival rate was determined using the Kaplan-Meier survival test, and cell viability was assessed using cell viability imaging kit and the MTT assay, and activation of the cell apoptosis pathway (Phosphorylated adenosine monophosphate activated protein kinase (p-AMPK), Apoptosis-inducing factor (AIF) and Caspase 3) were characterized by western blots. Hydrogen water administration improved the survival of mice with colon 26-induced cancer. Furthermore, hydrogen water enhanced cell apoptosis in cancer cells, resulting in a marked increase in the expression of p-AMPK, AIF and Caspase 3 in colon 26 cells. Hydrogen water also increased the inhibitory effect of 5-FU on colon 26 cells with spect to cell survival rate and anticancer functions. Additionally, high-content hydrogen water exhibited stronger antioxidative and anticancer activity than did the natural hydrogen water. In conclusion, high-content hydrogen water can inhibit colon cancer, particularly in combination with 5-fluorouracil.

PeerJ. 2015 Apr 7;3:e859. doi: 10.7717/peerj.859. eCollection 2015.


鹿児島大学大学院医歯学総合研究科 心身内科学



そのため本研究では、Colon-26がん細胞を用いたin vitroとin vivo 試験を両方行うことで、がんに対するH2と5-FUの相乗作用を調べました。